Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
Identifieur interne : 004D91 ( Main/Exploration ); précédent : 004D90; suivant : 004D92Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid
Auteurs : Li Baofeng ; Yuan Zhi ; Chen Bei [République populaire de Chine] ; Meng Guolin ; Yin Qingshui ; Liu JianSource :
- Acta Orthopaedica [ 1745-3674 ] ; 2010.
Abstract
Experimental models of osteoporosis in rabbits are useful to investigate anabolic agents because rabbits have an active Haversian remodeling and achieve skeletal maturiaty quickly. In this study, an experimental model of osteoporosis in rabbits induced by a combination of ovariectomy and glucocorticoid was characterized to provide a useful model for prevention and therapy of osteoporosis.
32 skeletally mature female rabbits were divided randomly into 4 groups: sham control, bilateral ovariectomy (OVX), 1mg/kg/day methylprednisolone (MP) for 8 weeks, and OVX in combination with MP. All rabbits were killed 10 weeks after surgery. Bone mineral density (BMD) of lumbar spine was measured by dual-energy X-ray absorptiometry at baseline, and at 6 and 10 weeks postoperatively. Bone microarchitecture and mechanical properties of lumbar vertebrae were investigated by high-resolution micro-computed tomography and compression test, respectively, after killing.
Mean BMD of the OVX+MP group at 10 weeks was reduced by about 36% from baseline (p < 0.001). Bone microarchitecture of lumbar vertebrae in the OVX+MP group indicated osteoporosis-associated deterioration. There was a statistically significant reduction in maximum load, stiffness, and energy absorption capacity of lumbar vertebrae in the OVX+MP group, but not in the OVX group, compared to the sham control. In the MP group, BMD and some microarchitecture parameters such as trabecular thickness and bone volume fraction were reduced. The mechanical properties were not statistically significantly different from those in the sham control group, however, although a negative trend was observed.
Osteopenia can be induced experimentally in rabbits through a combination of OVX and MP, and can be evaluated by BMD, bone microstructure, and mechanical parameters.
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DOI: 10.3109/17453674.2010.483986
PubMed: 20446884
PubMed Central: 2876847
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid</title><author><name sortKey="Baofeng, Li" sort="Baofeng, Li" uniqKey="Baofeng L" first="Li" last="Baofeng">Li Baofeng</name></author><author><name sortKey="Zhi, Yuan" sort="Zhi, Yuan" uniqKey="Zhi Y" first="Yuan" last="Zhi">Yuan Zhi</name><affiliation><nlm:aff id="AF0002"><institution>Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an</institution></nlm:aff></affiliation></author><author><name sortKey="Bei, Chen" sort="Bei, Chen" uniqKey="Bei C" first="Chen" last="Bei">Chen Bei</name><affiliation wicri:level="1"><nlm:aff id="AF0003"><institution>Department ofOncology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou</institution><country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Guolin, Meng" sort="Guolin, Meng" uniqKey="Guolin M" first="Meng" last="Guolin">Meng Guolin</name><affiliation><nlm:aff id="AF0002"><institution>Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an</institution></nlm:aff></affiliation></author><author><name sortKey="Qingshui, Yin" sort="Qingshui, Yin" uniqKey="Qingshui Y" first="Yin" last="Qingshui">Yin Qingshui</name><affiliation><nlm:aff id="AF0001"><institution>Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou</institution></nlm:aff></affiliation></author><author><name sortKey="Jian, Liu" sort="Jian, Liu" uniqKey="Jian L" first="Liu" last="Jian">Liu Jian</name><affiliation><nlm:aff id="AF0002"><institution>Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an</institution></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno 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type="wicri:Area/Main/Curation">004D91</idno><idno type="wicri:Area/Main/Exploration">004D91</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid</title><author><name sortKey="Baofeng, Li" sort="Baofeng, Li" uniqKey="Baofeng L" first="Li" last="Baofeng">Li Baofeng</name></author><author><name sortKey="Zhi, Yuan" sort="Zhi, Yuan" uniqKey="Zhi Y" first="Yuan" last="Zhi">Yuan Zhi</name><affiliation><nlm:aff id="AF0002"><institution>Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an</institution></nlm:aff></affiliation></author><author><name sortKey="Bei, Chen" sort="Bei, Chen" uniqKey="Bei C" first="Chen" last="Bei">Chen Bei</name><affiliation wicri:level="1"><nlm:aff id="AF0003"><institution>Department ofOncology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou</institution><country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Guolin, Meng" sort="Guolin, Meng" uniqKey="Guolin M" first="Meng" last="Guolin">Meng Guolin</name><affiliation><nlm:aff id="AF0002"><institution>Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an</institution></nlm:aff></affiliation></author><author><name sortKey="Qingshui, Yin" sort="Qingshui, Yin" uniqKey="Qingshui Y" first="Yin" last="Qingshui">Yin Qingshui</name><affiliation><nlm:aff id="AF0001"><institution>Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou</institution></nlm:aff></affiliation></author><author><name sortKey="Jian, Liu" sort="Jian, Liu" uniqKey="Jian L" first="Liu" last="Jian">Liu Jian</name><affiliation><nlm:aff id="AF0002"><institution>Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an</institution></nlm:aff></affiliation></author></analytic><series><title level="j">Acta Orthopaedica</title><idno type="ISSN">1745-3674</idno><idno type="eISSN">1745-3682</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background and purpose</title><p>Experimental models of osteoporosis in rabbits are useful to investigate anabolic agents because rabbits have an active Haversian remodeling and achieve skeletal maturiaty quickly. In this study, an experimental model of osteoporosis in rabbits induced by a combination of ovariectomy and glucocorticoid was characterized to provide a useful model for prevention and therapy of osteoporosis.</p></sec><sec><title>Methods</title><p>32 skeletally mature female rabbits were divided randomly into 4 groups: sham control, bilateral ovariectomy (OVX), 1mg/kg/day methylprednisolone (MP) for 8 weeks, and OVX in combination with MP. All rabbits were killed 10 weeks after surgery. Bone mineral density (BMD) of lumbar spine was measured by dual-energy X-ray absorptiometry at baseline, and at 6 and 10 weeks postoperatively. Bone microarchitecture and mechanical properties of lumbar vertebrae were investigated by high-resolution micro-computed tomography and compression test, respectively, after killing.</p></sec><sec><title>Results</title><p>Mean BMD of the OVX+MP group at 10 weeks was reduced by about 36% from baseline (p < 0.001). Bone microarchitecture of lumbar vertebrae in the OVX+MP group indicated osteoporosis-associated deterioration. There was a statistically significant reduction in maximum load, stiffness, and energy absorption capacity of lumbar vertebrae in the OVX+MP group, but not in the OVX group, compared to the sham control. In the MP group, BMD and some microarchitecture parameters such as trabecular thickness and bone volume fraction were reduced. The mechanical properties were not statistically significantly different from those in the sham control group, however, although a negative trend was observed.</p></sec><sec><title>Interpretation</title><p>Osteopenia can be induced experimentally in rabbits through a combination of OVX and MP, and can be evaluated by BMD, bone microstructure, and mechanical parameters.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Adinoff, Ad" uniqKey="Adinoff A">AD Adinoff</name></author><author><name sortKey="Hollister, Jr" uniqKey="Hollister J">JR Hollister</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cao, T" uniqKey="Cao T">T Cao</name></author><author><name sortKey="Shirota, T" uniqKey="Shirota T">T Shirota</name></author><author><name sortKey="Ohno, K" uniqKey="Ohno K">K Ohno</name></author><author><name sortKey="Michi, Ki" uniqKey="Michi K">KI 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last="Zhi">Yuan Zhi</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Bei, Chen" sort="Bei, Chen" uniqKey="Bei C" first="Chen" last="Bei">Chen Bei</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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